《植物生理学报》 2013, 49(8): 811-816

四翅滨藜AcERF1基因的克隆及其在酵母中的抗逆分析

孙新华**, 李敬涛**, 刘言志, 贾承国, 潘洪玉*

吉林大学植物科学学院, 长春130062 ** 共同第一作者

通信作者：孙新华；E-mail: panhongyu@jlu.edu.cn；Tel: 0431-85167420

摘　要：

ERF (ethylene responsive factor)转录因子是植物AP2/ERF超家族转录因子中一个重要的亚家族, 其DNA结合域可与多种病程相关基因启动子区的GCC-box特异性结合, 从而激发植物的抗逆应答反应。本研究通过筛选四翅滨藜(Atriplex canescens) cDNA文库, 获得一个ERF的全长cDNA序列, 命名为AcERF1 (Genbank序列号为JN984931), AcERF1基因开放阅读框为1 161 bp, 编码387个氨基酸, 其编码蛋白N端含有一个保守的类MCGGAI (I/L)基元。为了研究AcERF1基因的抗逆功能, 将其转入酵母进行盐、碱、干旱、高温、冷冻和活性氧等胁迫, 结果表明, 重组酵母在多种胁迫下的抗逆水平明显高于对照, 特别对高温、强碱和活性氧表现出明显抗性。推测该基因参与了四翅滨藜的抗逆调控过程。

关键词：四翅滨藜; 酵母表达; AcERF1; 抗逆

收稿：2013-05-14   修定：2013-07-07

资助：吉林省玉米产业技术体系(201203-1)和国家科技支撑计划项目(2012BAD19B04)

Cloning and Functional Characterization of An AcERF1 Gene from Atriplex canescens

SUN Xin-Hua**, LI Jing-Tao**, LIU Yan-Zhi, JIA Cheng-Guo, PAN Hong-Yu*

College of Plant Science, Jilin University, Changchun 130062, China

Corresponding author: SUN Xin-Hua; E-mail: panhongyu@jlu.edu.cn; Tel: 0431-85167420

Abstract:

The ethylene responsive factors (ERF) belong to a subfamily of the AP2/ERF superfamily in plants. The ERF subfamily were shown to specifically bind the GCC-box presented in many promoters of pathogenesis related protein (PR) genes, and implicated in various responses to environmental stimuli. In this study, a new member (AcERF1) of ERF subfamily was isolated from an Atriplex canescens cDNA library. The AcERF1 contains an open reading frame of 1 161 bp which encodes a protein of 387 amino acid residues with a conserved N-terminal MCGGAI (I/L) -like motif. To investigate its function associated with abiotic and biotic stress, AcERF1 was transformed into Saccharomyces cerevisiae INVSc1 and treated with saline, alkali, drought, high temperature, freezing, as well as paraquat, heterologous expression of AcERF1 in transgenetic yeast showed a relatively higher stress resistance, particularly in high temperature, alkali and paraquat conditions when compared with control. These results indicated that AcERF1 may involve in response to environmental stress and exhibit multiple, complex and flexible expression patterns in A. canescens.

Key words: Atriplex canescens; yeast expression; AcERF1; stress tolerance